Experimental investigation on growth profiles of microalgae with different flow conditions in photo-bioreactors
-
摘要: 高密度藻培对藻类资源高效利用十分重要。为解决流动条件对微藻生长作用机理不明的问题，本文借助高时间分辨率粒子图像测速技术（Time Resolved Particle Image Velocimeter, TR-PIV），对比研究光生物反应器(Photobioreactors, PBR)的水流速度分布特征和涡流效应，获得了藻液流速、涡量、湍流动能（TKE）云图，测量了小球藻的生长速率和类胡萝卜素含量。实验结果表明：高轴向速度、高径向速度、高涡量（0.015 s−1≤Ω≥0.025 s−1）、高TKE(k≤0.2 m2/s2)的流动会加速小球藻细胞的分裂、生长及高附加值产物产生；流场可视化方法是PBR设计与优化的一种有效工具。Abstract: Well-designed Photo-bioreactors (PBR) support high-efficiency biomass production. Methods: Flow fields of plat-plate PBR are experimental investigated with a time-resolved particle image velocimeter (TR-PIV). Results: Microalgae flow around sets of baffles improves performance of Chlorella vulgaris growth. Conclusion: The PIV-based flow visualization method benefits microalgae PBR designs, implements of high-density cultivations. The PBR structures proposed in this paper is of great supports to design and high productivity of bio-products with high added-values.
-
Key words:
- Photo-bioreactor /
- Flow visualization /
- TR-PIV /
- Microalgae /
- High density cultivation
-
图 3 PIV系统拍摄的YZ截面藻液瞬时流速
Figure 3. PIV captured velocity and streamline contours of algal liquid
图 4 PIV系统拍摄的不同曝气流量的藻液平均流速云图
Figure 4. PIV captured averaged velocity contours of algal liquid
图 5 对比PBR藻液脉动速度图
Figure 5. UY-axis and VZ-axis fluctuating velocities of algal liquids
图 6 PIV系统拍摄的PBR藻液轴向速度和径向速度
Figure 6. VZ-axis and UY-axis velocity profiles of algal liquids
图 7 PIV系统拍摄的藻液时均速度矢量云图
Figure 7. PIV captured velocity vector contours of algal liquid
图 10 PIV系统拍摄的时均湍流动能云图
Figure 10. PIV captured turbulent kinetic energy contours of algal liquid
图 12 流动影响参数a与藻生长、生物质产率的关联图
Figure 12. Relationship between a, algae growth, biomass productivity
表 1 小球藻培养结果
Table 1. Chlorella vuguris cultivation data
培养时间/天 I/A 1# PBR 2# PBR 3# PBR 0 0.179 0.179 0.179 2 0.169 0.353 0.303 4 0.202 1.124 0.962 6 0.245 2.332 2.240 7 0.225 3.220 3.036 8 0.233 4.963 4.473 10 0.342 6.083 5.684 
下载: 导出CSV
-
[1] 李岩溪. 浅层跑道池培养微藻的驱动和混合强化[D]. 北京: 中国科学院大学, 2014.LI Y X. Driving and mixing enhancement of microalgae culture in shallow runway pool[D]. Beijing: University of Chinese Academy of Sciences, 2014. [2] WANG X X, ZHANG T Y, DAO G H, et al. Assessment and mechanisms of microalgae growth inhibition by phosphonates: effects of intrinsic toxicity and complexation[J]. Water Research, 2020, 186: 116333. doi: 10.1016/j.watres.2020.116333 [3] 张文毓. 生物柴油的现状与进展[J]. 生物技术世界, 2008, 5(4): 25–27. [4] 黄鹏, 田腾飞, 张文安, 等. 水动力条件对水体中藻类生长的抑制作用[J]. 环境工程, 2018, 36(12): 64–69.HUANG P, TIAN T F, ZHANG W A, et al. Inhibition of algae growth in water by hydroynamic force[J]. Environmental Engineering, 2018, 36(12): 64–69. [5] 吴晓辉, 李其军. 水动力条件对藻类影响的研究进展[J]. 生态环境学报, 2010, 19(7): 1732–1738. doi: 10.3969/j.issn.1674-5906.2010.07.039WU X H, LI Q J. Reviews of influences from hydrodynamic conditions on algae[J]. Ecology and Environment, 2010, 19(7): 1732–1738. doi: 10.3969/j.issn.1674-5906.2010.07.039 [6] 杨宗波. 闪光反应器流场优化促进微藻固定燃煤烟气CO2研究[D]. 杭州: 浙江大学, .YANG Z B. Optimization of flow field in flash reactor promotes CO2 fixation of coal-fired flue gas by microalgae. [D]. Hangzhou: Zhejiang University. [7] SANGEETHA T, LI I T, LAN T H, et al. A fluid dynamics perspective on the flow dependent performance of honey comb microbial fuel cells[J]. Energy, 2021, 214: 118928. doi: 10.1016/j.energy.2020.118928 [8] SEBASTIÁN M, GASOL J M. Visualization is crucial for understanding microbial processes in the ocean[J]. Philosophical Transactions of the Royal Society B:Biological Sciences, 2019, 374(1786): 20190083. doi: 10.1098/rstb.2019.0083 [9] WONG Y K, HO K C, TSANG Y F, et al. Cultivation of Chlorella vulgaris in column photobioreactor for biomass production and lipid accumulation[J]. Water Environment Research:a Research Publication of the Water Environment Federation, 2016, 88(1): 40–46. doi: 10.2175/106143015X14362865227553 [10] CHOWDURY K H, NAHAR N, DEB U K. The growth factors involved in microalgae cultivation for biofuel production: a review[J]. Computational Water, Energy, and Environmental Engineering, 2020, 9(4): 185–215. doi: 10.4236/cweee.2020.94012 [11] WONG Y K , HO K C, LAI P K, et al. PBRs for Cultivation of Microalgae: Assessment of Design and Performance[C]//Proc of ICBUAB 2013. 2013. [12] DETRELL G. Chlorella vulgaris photobioreactor for oxygen and food production on a moon base—potential and challenges[J]. Frontiers in Astronomy and Space Sciences, 2021, 8: 700579. doi: 10.3389/fspas.2021.700579 [13] SFORZA E, ENZO M, BERTUCCO A. Design of microalgal biomass production in a continuous photobioreactor: an integrated experimental and modeling approach[J]. Chemical Engineering Research and Design, 2014, 92(6): 1153–1162. doi: 10.1016/j.cherd.2013.08.017 [14] ASLANBAY GULER B, DENIZ I, DEMIREL Z, et al. Computational fluid dynamics modelling of stirred tank photobioreactor for Haematococcus pluvialis production: Hydrodynamics and mixing conditions[J]. Algal Research, 2020, 47: 101854. doi: 10.1016/j.algal.2020.101854 [15] BERTUCCO A, BERALDI M, SFORZA E. Continuous microalgal cultivation in a laboratory-scale photobioreactor under seasonal day-night irradiation: experiments and simulation[J]. Bioprocess and Biosystems Engineering, 2014, 37(8): 1535–1542. doi: 10.1007/s00449-014-1125-5 [16] HADIYANTO H, ELMORE S, VAN GERVEN T, et al. Hydrodynamic evaluations in high rate algae pond (HRAP) design[J]. Chemical Engineering Journal, 2013, 217: 231–239. doi: 10.1016/j.cej.2012.12.015 [17] HUANG J, XI B D, XU Q J, et al. Experiment study of the effects of hydrodynamic disturbance on the interaction between the cyanobacterial growth and the nutrients[J]. Journal of Hydrodynamics, 2016, 28(3): 411–422. doi: 10.1016/s1001-6058(16)60644-3 [18] YANG Z F, DEL NINNO M, WEN Z Y, et al. An experimental investigation on the multiphase flows and turbulent mixing in a flat-panel photobioreactor for algae cultivation[J]. Journal of Applied Phycology, 2014, 26(5): 2097–2107. doi: 10.1007/s10811-014-0239-0 [19] SCHRIMPF M, ESTEBAN J, WARMELING H, et al. Taylor-couettereactor: principles, design, and applications[J]. AIChE Journal, 2021, 67(5). doi: 10.1002/aic.17228 [20] 董亮, 曾涛, 刘少北, 等. 生物倍增反应器气泡流态特性分析[J]. 水利水运工程学报, 2017(4): 67–75.DONG L, ZENG T, LIU S B, et al. PIV measurement and POD analysis of bubble flow characteristics in bio-doubling reactor[J]. Hydro-Science and Engineering, 2017(4): 67–75. [21] 黄娜, 陈思晔, 齐瀚实. 短期连续剪切对光生物反应器内海带配子体细胞生长及其恢复能力的影响[J]. 生物工程学报, 2007, 23(5): 935–940. doi: 10.3321/j.issn:1000-3061.2007.05.031HUANG N, CHEN S Y, QI H. Effects of short-term continuous shear stress on cells growth and recovery of laminaria japonica gametophytic cells in photobioreactor[J]. Chinese Journal of Biotechnology, 2007, 23(5): 935–940. doi: 10.3321/j.issn:1000-3061.2007.05.031 [22] BAZDAR E, ROSHANDEL R, YAGHMAEI S, et al. The effect of different light intensities and light/dark regimes on the performance of photosynthetic microalgae microbial fuel cell[J]. Bioresource Technology, 2018, 261: 350–360. doi: 10.1016/j.biortech.2018.04.026 [23] BITOG J P P, LEE I B, OH H M, et al. Optimised hydrodynamic parameters for the design of photobioreactors using computational fluid dynamics and experimental validation[J]. Biosystems Engineering, 2014, 122: 42–61. doi: 10.1016/j.biosystemseng.2014.03.006 [24] 齐祥明, 崔海龙. 基于CFD数值模拟的平板式微藻光生物反应器比较[J]. 农业工程学报, 2015, 31(13): 215–221.QI X M, CUI H L. Comparison of flat photo-bioreactors for micro-algae culture based on CFD numerical simulation[J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(13): 215–221. [25] HUANG J K, KANG S F, WAN M X, et al. Numerical and experimental study on the performance of flat-plate photobioreactors with different inner structures for microalgae cultivation[J]. Journal of Applied Phycology, 2015, 27(1): 49–58. doi: 10.1007/s10811-014-0281-y [26] MICHELS M H A, VAN DER GOOT A J, NORSKER N H, et al. Effects of shear stress on the microalgae Chaetoceros muelleri[J]. Bioprocess and Biosystems Engineering, 2010, 33(8): 921–927. doi: 10.1007/s00449-010-0415-9 [27] PUHALES F S, DEMARCO G, MARTINS L G N, et al. Estimates of turbulent kinetic energy dissipation rate for a stratified flow in a wind tunnel[J]. Physica A:Statistical Mechanics and Its Applications, 2015, 431: 175–187. doi: 10.1016/j.physa.2015.03.008 [28] ARAJI M T, SHAHID I. Symbiosis optimization of building envelopes and micro-algae photobioreactors[J]. Journal of Building Engineering, 2018, 18: 58–65. doi: 10.1016/j.jobe.2018.02.008 [29] SCHEUFELE F B, LARISSA HINTERHOLZ C, ZAHARIEVA M M, et al. Complex mathematical analysis of photobioreactor system[J]. Engineering in Life Sciences, 2019, 19(12): 844–859. doi: 10.1002/elsc.201800044 -
点击查看大图
图(12) / 表(1)
计量
- 文章访问数: 77
- HTML全文浏览量: 133
- PDF下载量: 16
- 被引次数: 0
