Abstract: In order to study the hemodynamics of the coronary artery in vitro and to understand the mechanism of cardiovascular disease from the perspective of fluid mechanics, a mock circulatory loop with systemic circulation and coronary circulation was constructed. Combined with a variety of fluid measurement techniques, the hemodynamics of the benchmark nozzle model proposed by Food and Drug Administration (FDA) was studied in vitro under the condition of coronary flow. The location of thrombus in the nozzle model was qualitatively predicted by the platelet adhesion emulation technique using fluorescent particles. The flow field inside the nozzle model was captured by particle image velocimetry (PIV), and the relationship between the thrombus formation and hemodynamics at the corresponding location was quantitatively analyzed. The results show that fluorescent particles are easy to adhere to the wall of the model near the flow structure of the backward-facing step, and the flow field data show that the thrombus formation location is related to the low velocity region and reflux near the wall. In vitro platelet adhesion emulation and hemodynamic research can provide references for coronary thrombotic investigation and related medical device development.