Abstract: The cryogenic wind tunnel is the best way to achieve flight Reynolds number testing in engineering application, with great strategy importance in the national defense field. The insulation structure has become one of the key technologies for building large cryogenic wind tunnels. On this account, design and property analysis on insulation structure is investigated with the help of numerical simulation and experiment. Firstly, considering the wind tunnel working condition, design and material selection for the insulation structure is carried out. Then, the finite element modeling of the insulation structure is built. On this basis, the property of the insulation structure is numerically studied based on the actual worst working condition of the wind tunnel. The insulation characteristics and stress/displacement distribution of the insulation structure are analyzed. Finally, the experimental platform able to simulate the working condition of cryogenic wind tunnel is designed and constructed to carry out the temperature and stress/strain measurement experiment for the insulation structure under cryogenic alternating pressure working condition. The obtained results show that the designed insulation structure satisfies the demands of the cryogenic wind tunnel in service, which solves the problem of insulation structure designation. Additionally, the designed experimental platform solves the problem of property evaluation of the insulation structure, which paves the way for building the cryogenic wind tunnel of our country in the future.