Abstract: In order to study motion characteristics of the separation between the cruise stages and booster stages, it is needed to develop the device of the dynamic stage separation for the dynamic stage separation wind tunnel test. Hypervelocity aerodynamics institute of CARDC studied and developed the dynamic separation device aimed at application in routine hypersonic wind tunnels, which includes the support system of dynamic separation and the dynamic stage separation support and release device. In the design, the finite element analysis is used to evaluate the characteristics of the support structure. The analysis contains the inherent frequency analysis. The vision measurement system is used to measure the trajectory and the runtime of the dynamic stage separation support and release device. The runtime is 78 ms, which satisfies the requirement of the wind tunnel test. Finally a series of wind tunnel tests were conducted in the hypersonic wind tunnel. The results of computation and the wind tunnel test indicate that: the inherent frequency of the support structure, and the runtime of the dynamic stage separation support and release device, which is 78 ms, can satisfy the need of the wind tunnel. According to the different particular wind tunnel circumstances, the size of the dynamic separation device can be reformed, which can be applied to different hypersonic wind tunnels.