Abstract: In this study, a system was designed and built up for the pressure measurement along micro/mini-channel, which mainly includes micro/mini-channel and square pressure cavities on PMMA chip, micro-strain sensors and multi-channel strain instruments. The static pressure in micro/mini-channel was provided by the syringe pump, and the high precision pressure values measured by the micromanometer of FCO510 was employed as the standard pressure. The strain values from the strain sensors installed on the square pressure cavities were obtained by multi-channels strain gauge, and the calibration functions between the standard pressure and the strain were established. The pressure distributions of three kinds of micro pressure chips were measured under flow rates of 80, 70, 60 and 50mL/min, respectively. The pressures have good linear distribution.The uncertainty analysis indicates that the relative uncertainty of the pressure error is between 0.15% and 6.82%. The validity and reliability of pressure measurement are high.